Antioxidant Grape Seed extract
Pulished on Aug. 22, 2019
Grape seed extract：
Antioxidant Grape Seed extract is a kind of polyphenols extracted from grape seeds, mainly composed of procyanidins, catechins, epicatechins, gallic acid, epicatechin gallate and other polyphenols.
Characteristics of grape seed extract:
Grape seed extract is a pure natural substance. It is one of the most effective antioxidants from plant sources found to date. Tests show that its antioxidant effect is 30 to 50 times that of vitamins C and E.
Procyanidins have a strong activity, can inhibit carcinogens in cigarettes, in the water phase of free radical trapping ability is 2 ~ 7 times of the general antioxidant, such as alpha-tocopherol more than twice the activity.
Grape Seed Extract
Extract of China Grape Seed Extract：
Studies have found that in many plant tissues, the content of procyanidins in grape seeds and pine bark extracts is the highest, and the extraction methods of procyanidins from grape seeds mainly include solvent extraction, microwave extraction, ultrasonic extraction and supercritical CO2 extraction. There are many impurities in grape seed procyanidins extract, which need further purification to improve the purity of procyanidins. Common purification methods include solvent extraction, membrane filtration and chromatography.
Ethanol concentration had the most significant effect on the extraction rate of grape seed procyanidins, while the extraction time and temperature had no significant effect on the extraction rate of grape seed procyanidins. The optimal extraction process parameters as follows: 70% ethanol concentration, extraction time, 120 m in the solid-liquid ratio 1:20.
Static adsorption experiments showed that HPD - 700 of procyanidins adsorption rate up to 82.85%, followed by DA201, to 82.68%, the difference were similar, and these two kinds of resin adsorption quantity of procyanidins are the same; In desorption experiment, DA201 resin of procyanidins desorption rate is highest, for 60.58%, and the desorption rate of HPD - 700 of procyanidins. Only 50.83%. DA210 resin was determined to be the best adsorption resin for the separation of procyanidins.
Through process optimization, the concentration of procyanidins 0-15 mg/mL, the flow rate of 1 mL/m in, with 70% ethanol solution as the eluent, flow rate of 1 mL/m in, dosage of eluent for 5 bv, can extract of grape seed procyanidins preliminary purification.
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